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Figure 1. Binding assay. Competition of various steroids for binding of 3H cortisol to the hGR in HeLa cells. Binding data are expressed as the percentage of specific binding (Bs) remaining after adding increasing amounts of <t>competitor.</t>
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Figure 1. Binding assay. Competition of various steroids for binding of 3H cortisol to the hGR in HeLa cells. Binding data are expressed as the percentage of specific binding (Bs) remaining after adding increasing amounts of competitor.

Journal: Endocrinology

Article Title: Adrenal Steroid Metabolites Accumulating in Congenital Adrenal Hyperplasia Lead to Transactivation of the Glucocorticoid Receptor.

doi: 10.1210/en.2015-1087

Figure Lengend Snippet: Figure 1. Binding assay. Competition of various steroids for binding of 3H cortisol to the hGR in HeLa cells. Binding data are expressed as the percentage of specific binding (Bs) remaining after adding increasing amounts of competitor.

Article Snippet: Whole cells (0.2–1.0 106) were incubated in serum and phenol-red-free RPMI medium (final volume, 150 L) for 1.5–2 hours at 37°C in a series of 0.5 mL microcentrifuge tubes containing 30nM 3H-cortisol (PerkinElmer, Inc.) and an increasing amount of unlabeled competitor: cortisol, 17OHP, 21DF, 4 (all Steraloids) or P (Sigma-Aldrich).

Techniques: Binding Assay